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The selected areas were searched during consecutive nights Sexy women in oulu detect females that were glowing for the first time. These were brought to the laboratory, kept on a light rhythm that approximated natural conditions and allowed to mate. The number of eggs laid was counted, and the ventral side of the females with the lantern was photographed after death. The presence of fully grown eggs left in the ovaries was checked through dissection. Female mating status and the number of eggs laid before capture were unknown. However, females stop glowing after mating [ 9 ] and it is consequently unlikely Sexy women in oulu they had laid any eggs before capture.
All females stopped glowing after mating in the laboratory. Lantern surface area was measured using ImageJ v. It was correlated to fecundity using Pearson correlation, using both the number of laid eggs and the total number of eggs by including unlaid eggs. The results did not differ and we present only results for number of eggs laid. To test whether lantern size correlates with brightness, we estimated the brightness of captive females on a five-point scale 1, barely visible; 5, very bright. The risk of observer bias was low as the identity of individuals could not be determined in the dark laboratory and as the individuals changed location each day.
We used Spearman's rank correlation to test for a correlation between female brightness, lantern size and number of eggs. We chose the brightest glow of each female, because brightness declines with age G. Baudryunpublished data. We used pairs of traps with differing light intensity 1 m apart. Each trap was made of a 1. At the top of each trap was a green 3 mm through-hole LED similar in wavelength nm to a female glow-worm — nm [ 916 ]. We placed two to five trap pairs in different locations each night. We varied the brightness of the traps by wiring varying amounts of ohm resistors to the LEDs. Two different combinations of resistors were used: The brightness of the lights cannot be directly compared using resistance, as the total resistance in the two trap pairs was different ohm versus ohm.
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These differences Sexu relative brightness were reflective of the range found among real Sexy women in oulu J. Hopkinspersonal observations. The traps were placed in the same areas where females had been collected. Trapping was conducted in June and July The position of each trap in the pairs was chosen arbitrarily each night. Traps were not set during nights with high wind or rain when males are less oul to be active. We used a nested ANOVA to test whether males preferred brighter light, with the number of males per trap each night as the dependent variable, and the two types of trap pairs and the two brightnesses of traps in them as fixed factors brightness nested inside type of pair.
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